Operator training system for the fermentation and cell lysis stages of the recombinant Streptokinase production process

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Abstract

The growing incidence of acute myocardial infarction has positioned recombinant streptokinase as an essential therapy. In Cuba, the Center for Genetic Engineering and Biotechnology developed the Cuban recombinant streptokinase (Heberkinasa ER), whose production at the National Biopreparations Center involves critical stages such as fermentation and cell disruption. The recent resumption of production, combined with the incorporation of new operators, has created training challenges due to specialist shortages and the absence of a systematic plan. To address this problem, this work presents a training system, framed within educational software, to train operators in the fermentation and cell disruption stages. The main objective is to evaluate operator decision-making in critical situations, providing the lead technologist with information about their performance and learning progress. For this purpose, was designed a system that integrates a knowledge base with simulated scenarios, allowing operators to practice and receive immediate feedback. During the testing phase, the states of 45 variables, 98 causes, and 121 recommendations were evaluated. The results demonstrated the correspondence between user responses and the logic established in the inference trees, validating the system's effectiveness in identifying correct and incorrect responses. This system, based on knowledge base management tools and operator training for the food industry, can be used in any case with similar process characteristics.